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During our previous characterization of molecular pathways leading to wheat resistance to Hessian fly using microarrays, we have found that the transcript levels of a large number of genes encoding various enzymes involved in glutathione metabolism are strongly affected in resistant plants after Hessian fly attack 21,27 , indicating that glutathione may play an important role in wheat defense against Hessian fly attack

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Next, the cells were rewashed three times with fresh RPMI1640 medium and observed by fluorescent microscopy (F6000, Leica Microsystems)

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