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Vigorous shaking denatures the peptide, breaking the molecular bonds that make it functional

10.3748/wjg.v22.i41.9127 15 DrmicD.KolencD.IlicS.BaukL.SeverM.SeverZ.et al (2017)

Self-amplifying mRNA generates high and more durable expression of the encoded coronavirus antigens, which could translate to greater immunogenicity than nonamplifying mRNA vaccines [155, 156]