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The cytosolic Grx1-roGFP2 and to a lesser extent the plastidic TKTP-Grx1-roGFP2 lines exhibited lower fluorescence intensity when excited at 488 nm after 24 h or 48 h of AITC treatment compared to their respective control, indicating oxidative conditions and/or lower levels of glutathione (Fig

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These compounds are formed continuously as byproducts of aerobic metabolism, through reactions with drugs and environmental toxins, or when the level of antioxidants is diminished, all creating the condition of oxidative stress