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To isolate chondrocytes, fresh articular cartilage tissues were rinsed with basal medium (BM), consisted of high glucose Dulbeccos modified Eagles medium (DMEM, Gibco/Thermo Fisher Scientific, Waltham, MA, United States), with 2 Antibiotics-Antimycotics (anti-anti, Life Technologies, Carlsbad, CA, United States) and then cut into 1 mm 3 pieces

The latter regulates the Nrf2-ARE pathway mainly through phosphorylation sites and is considered a critical regulatory factor of Nrf2 nuclear accumulation, nuclear rejection, and degradation

doi:10.1126/science.289.5484.1567

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