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Despite advancing our understanding of plant responses to cold stress, we remain insufficiently informed about the interaction of various systems governing cold tolerance and how this knowledge can be applied to increase crop resilience (Roychowdhury et al., 2023b)

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Despite these advantages, many current HILIC methods still rely on chemical derivatization to achieve adequate sensitivity [48, 59,60,61] or depend entirely on MS/MS specificity while neglecting optimal chromatographic separation, which can lead to severe matrix interferences and ion suppression [41]

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