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For immunohistochemistry and immunoprecipitation studies, mice were deeply anesthetized with isoflurane (2% pure oxygen) and transcardially perfused with PBS to remove blood

More recent literature has revisited the fragment in the context of combination studies, where researchers pair it with secretagogues to separate direct adipose effects from endogenous GH-axis activation

Side Effect Comparison Both agents share gastrointestinal side effects as their primary tolerability issue

Low levels of magnesium can be seen as fatigue, involuntary muscle contractions, disorientation, convulsions, dizziness or increased heart rate