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Cells were then stained with CD45 (Biolegend, #103155) and Liperfluo (described above) for 30 minutes at 37C, washed 3 times with PBS, analyzed on an LSRII flow cytometer using the CD45 population as a proxy for tumor cells, and normalized to mean fluorescence intensity of control samples

Without extra vitamin B12, the combination of pernicious anemia and menstruation could be dangerous and have long-term consequences