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Metabolite intermediates or other specific macromolecules leverage porins and outer membrane transporters (TOM) to cross the mitochondrial membrane into the inner mitochondrial space (IMS) or mitochondrial matrix, regulating the TCA cycle and oxidative respiratory chain [20]

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Following removal of TNF-containing medium, CM from these C2C12 cultures was collected and used to treat AML12 hepatocytes in combination with PA administration (Fig