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For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig

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Why the Neck Ages Faster Than the Face The neck has: Thinner dermal structure Fewer oil glands Constant movement High sun exposure Rapid collagen depletion after 35 By age 40+, collagen production can decline by up to 1% per year