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For IGF-1 LR3 specifically, the IGF-1 LR3 dosage calculator walks through the same math

For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method

Tissue debris were then filter out through strainer, yielding a solution containing various cell types, which were subsequently pelleted through centrifugating at 200g for 5 min

For a deeper primer on what peptides are and how they act, see what do peptides do, are peptides safe and are peptides steroids