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Even when hyperglycemia is managed, diabetic problems may still arise, because hyperglycemia causes mitochondria to produce too many ROS [40]
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Shortly, two methods were applied for separate extraction of lipids and polar/semipolar metabolites and the extracts were then analyzed using an ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) as described previously [39] and the data were processed using MZmine 2.53 [45]
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