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Hold the vial upright on your work surface and pierce the rubber stopper at a 45-degree angle, directing the needle tip toward the vial wall rather than straight down onto the lyophilised powder

c , Immunoblot of indicated proteins in the indicated HEK-293T SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with the indicated sgRNA

State clearly that results are based on animal-to-human scaling

Since the cloning of the first OR, those models have been superseded by heterologous expression systems (HEK, CHO, COS-7, Xenopus laevis oocyte) in which OR are easily expressed in large amount but whose cellular characteristics are far from neurons in which OR are endogenously expressed