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That single fractionation study is the proof point researchers still cite more than fifty years later as the origin of the entire copper-peptide research field: a compound identified almost by accident in a liver-aging comparison went on to become one of the most studied metallopeptides in dermal and regenerative research

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Lactate levels in culture medium, BALF, fibroblasts and lung tissue homogenates were measured using a Lactate Assay Kit (Jiancheng Bioengineering Institute, Nanjing, China)