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For differential expression analysis using the R package limma (27) (see below, section Comparison of group means), 2 parameters estimated by RUVs were included as covariates in the linear model, while the normalized logCPM data without RUVs correction was used as expression matrix input, as is recommended by the authors of the RUVSeq package for this type of analysis

In mammalian cells, APE1 also has a unique REDOX function, directing the activity of different transcription factors, thereby affecting gene expression and protein production [22]