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Acetylation modulates N-terminal autoproteolytic processing of SnCE1 The data shown above indicate lysine acetylation in wild type SnCE1 prohibits proteolytic processing, while all non-acetylated mutants, including SnCE1 Y212A and SnCE1 C256A, show proteolytic processing as visible by immunoblotting with a specific anti-SnCE1 antibody (Figs

However, this is usually mild and will wear off relatively quickly

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112 More recently, it was suggested that methotrexate causes ferroptosis through activation of autophagy, leading to increased degradation of ferritin and liberation of ferrous iron (ferritinophagy), which promotes LPO and liver injury