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Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development

In parallel, chronic ethanol exposure in mice impairs dendritic cell presentation of exogenous antigens through MHC II and alters costimulatory profiles, leading to diminished antigenspecific Tcell activation despite largely preserved MHC I crosspresentation (54)