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First, although we identified significant alterations in pathways such as fatty acid -oxidation and PPAR signaling, we lacked direct functional validation through experiments such as mitochondrial respiration assays, gene expression analysis of key regulators (e.g., PPAR target genes), or isotopic tracing of fatty acid oxidation

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Transcriptional regulation of miRNAs has been reported in a few studies and it is one of the encouraged topics to be investigated for the role of miRNAs