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Personalized dosing algorithms Developing predictive models to identify optimal escalation schedules based on individual characteristics

Because this article has 11 major sections, scan the headings first and then use the FAQ or summary sections to pressure-test the answer

Minimise headspace and stopper-open time, keep solutions cold, and prepare single-use aliquots rather than repeatedly accessing one vial

The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)