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The p Ka of the putative catalytic cysteine is lower in GRXC2 than in ROXY9 To investigate the potential reactivity of Cys A of ROXY9 and GRXC2 as a nucleophile under physiological p H , we compared the apparent p K a values of the cysteines of strep-MBP-ROXY9 and strep-MBP-GRXC2