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Simultaneously, CS suppresses the transcription of pro-inflammatory genes by inhibiting transcription factors like nuclear factor-B (NF-B) and activator protein 1 (AP-1), which are responsible for the expression of cytokines, chemokines, and other inflammatory mediators (Patil et al

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For experiments involving alterations to the culture medium, cells were seeded in regular RPMI 1640 with 5% dialysed FBS (Gibco, 26400044) and 1% penicillin and streptomycin