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Advancements in next-generation sequencing and protein expression profiling have demonstrated that the renal targets of thiazides, i.e., Na + Cl cotransporter (NCC, encoded by SLC12A3 ) and that of bumetanide/furosemide, i.e., Na + K + 2Cl cotransporter-2 (NKCC2, encoded by SLC12A1 ) are expressed in different tissues and cells, albeit at lower levels (Di Fulvio and Alvarez-Leefmans, 2009)

FIGURE 1 Drug abuse effects on metabolism and epigenetics The metabolic syndrome is a collection of metabolic abnormalities, including hyperinsulinemia, hypertension, dyslipidaemia, and abdominal obesity, and may be triggered by initial discrepancies at the cellular level in critical metabolic pathways

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