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and cool, wet winters (JuneAugust) with maximum average rainfalls of 300 mm (Archer et al

RNA isolation and quantitative RT-PCR Total RNA from cells was isolated with Trizol reagent (Qiagen GmbH, Hilden, Germany) and RT-PCR was conducted using a quantitative SYBR Green RT-PCR kit (Applied Biosystems, Warrington, UK) was used with a Step One Plus RT-PCR System (Applied Biosystems)

HCT116 and SW837 cells were pre-treated with metformin (10 mM) or H 2 O vehicle control for 24 h, exposed to 1.8 Gy radiation, and the cell cycle distribution assessed at 6 h, 10 h and 24 h post irradiation

The susceptibility to these effects can vary among animals based on factors, such as age, sex, health condition, and method of dose administration