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High levels of ROS, excessive inflammatory cells and cytokines in the wound microenvironment, insufficient new blood vessels, and weakened cell proliferation are the key factors that lead to the difficult healing of diabetic wounds 24

Direct intravenous NAD+ is tested at doses of 500 to 1000 mg infused over 2 to 6 hours

To determine CI activity (NADH-dependent OCR), MAS plus alamethicin (2.5 g ml 1 ) and cytochrome c (10 g ml 1 ) was added onto the cells followed by 5 mM pyruvate + 5 mM malate + 1 mM NADH + 10 nM recombinant perfringolysin O at port A, 2 M rotenone + 4 M antimycin A at port B, 0.5 mM TMPD + 1 mM ascorbic acid at port C and 50 mM azide at port D

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