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The diagnosis of acute cholecystitis relies on the history of risk factors, the assessment of clinical manifestations, laboratory tests, and imaging [123, 124]

The experimental endpoint was 250 days of age unless palpable tumors were detected

Proteins were then subjected to digestion with 250 ng trypsin (Promega) overnight at 37 C at a 50:1 protein:trypsin ratio, followed by a second trypsin digestion (250 ng) for 4 h

Despite the absence of a statistically significant doseresponse relationship observed in this analysis, clinical decision-making should consider that absorption efficiencyrather than absolute dosagemay be the limiting factor