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Doses are adjusted as needed over time

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coli strains harboring egt1 and egt2 from the fungus Trichoderma reesei achieved EGT production of 4.34 g/L in 143 h of fed-batch cultivation with constant supplementation of the mixture of precursor amino acids L-histidine, L-methionine, and L-cysteine (40 g/L each) after induction of expression of egt1 and egt2 (Chen et al