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Subsequently, the sections were incubated with secondary antibody (goat anti-rabbit), rinsed with phosphate buffer saline (PBS), stained with DAB, and hematoxylin and eosin (H&E) staining, dehydrated, transparentized, and mounted before microscopic analysis

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These innovations have contributed to a paradigm shift toward safe and effective, but less invasive, procedure-based treatment in addressing medical and aesthetic concerns in dermatology