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As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins

These medications, including ibuprofen, naproxen, and aspirin, block enzymes called COX-1 and COX-2 throughout the body

Semaglutide doses were selected based on previously validated neuroprotective and metabolic studies, while the papaya dose was chosen to reflect a nutritionally relevant antioxidant intake without inducing caloric overload

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