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The slices were incubated for at least 1 hour at 34C in ACSF composed of 126 mM NaCl, 2.8 mM KCl, 26 mM NaHCO 3 , 1.25 mM NaH 2 PO 4 , 1.2 mM MgSO 4 , 2.5 mM CaCl 2 , and 5 mM glucose (pH 7.3), while being continuously bubbled with 95% O 2 and 5% CO 2