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Isolated islets were cultured in RPMI 1640 medium (Hyclone, South Logan, UT, USA) with 11.1 mM glucose (Solarbio, Beijing, China) and 10% fetal bovine serum (Gibco, Grand Island, NY, USA) supplemented with 1% penicillin and streptomycin under 37 C and 5% CO 2 conditions

Vitamin B 12 can also be absorbed by passive diffusion, but this process is very inefficient only about 1% to 2% of the oral vitamin B 12 dose is absorbed passively (2)

2012, doi: 10.1016/j.ghir.2012.05.002