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P53 protein levels fluctuate across the day, driven by circadian control of MDM2 (the E3 ubiquitin ligase that targets p53 for degradation)

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Additionally, a similar experiment was completed by combining C159S (58 M) and a range of NADPH concentrations (9.5309 M) and monitoring the reaction at 445 and 600 nm to determine how the two phases observed titrate with increasing NADPH concentration