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Chromatographic conditions employ a C18 reversed-phase column, with acetonitrilewater solution (adjusted to an appropriate acidity with phosphoric acid) as the mobile phase

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However, Mdv1 has not been detected in mammalian cells (Stojanovski et al., 2004), where FIS1 is required to interact with additional outer membrane receptors, including Mff, MID49, and MID51, to facilitate Drp1 polymerization and assembly into a helical or ring structure surrounding the mitochondrial outer membrane, thereby recruiting Drp1 to the division site, which ultimately initiates fission (Takamura et al., 2012)