For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method
De esta manera, aumenta la permeabilidad de la piel y del tejido conectivo, se induce la angiognesis y se liberan factores de crecimiento en la matriz extracelular
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therefore, device handling errors are reduced among elderly patients with COPD