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Studies have found that berberine attenuates ischemia-reperfusion injury through NF-B nuclear translocation in mice with transient cerebral artery occlusion, however, this neuroprotective effect was more pronounced at the high dose (50 mg/kg) than the low dose (25 mg/kg) (Zhu et al., 2018)

The real frontier is delivery science and understanding that a compound is only as good as your ability to actually get it into the cell

Nature Reviews Endocrinology, 11, 535546

If the injection is to be given into the gluteus medius muscle, it will need to be carried out by a qualified nurse exercising extra caution, to avoid touching the sciatic nerve