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Roberto GM, Paiva HH, Botelho de Souza LE, et al

If the concentration changed, your unit count must change too

Statistical analyses The sample size calculation is based on the normal approximation of the test statistic for comparing two means assuming a mean difference of 0.6% [18], a standard deviation of 1.27%, a power of 80%, a two-sided significance level of of 0.05 and a drop-out rate of 15%