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Following induction, cells were harvested by centrifugation at 7500 rpm for 10 min at 4 C, and the resulting pellet was washed twice with 50 mL TB to remove residual IPTG

But you can also dose up through shots or supplements

Another case report introduces an AGEP-like reaction following semaglutide injection that did not present with pustules [8], further emphasizing the importance of maintaining a broad differential when investigating cutaneous eruptions in patients taking these medications, as they could present differently than the classical clinical picture