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It was first discovered by the Johnstone team in 1983 that these small particles were associated with the release of transferrin receptors during the maturation of sheep reticulocytes ( The release of sEVs requires several cellular steps, including the generation of intraluminal vesicles (ILVs) from MVBs, fusion of MVBs with the plasma membrane, and sorting of distinct sEV cargoes ( Figure 1 , sEVs originate from the endosomal pathway by the formation of early endosomes and late endosomes/MVBs

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However, these usually do not produce the tarry texture or strong odour associated with melena