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The cells were transiently co-transfected with the expression vectors encoding heavy chain and light chain using 293fectin TM (Invitrogen) in serum-free FreeStyle 293 Expression Medium (Gibco)

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doi: 10.1113/JP272613 59 DonohoeDRGargeNZhangXSunWO'ConnellTMBungerMKet al

These effects were particularly notable in the context of overexpression of miR-339-5p following brain injuries such as bleeds and strokes, underscoring GHK-Cu's potential as a research subject in the context of neurodegeneration