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After addition of 10 l Premix WST-1 solution to each well, cells were incubated at 37C for another 1 hour and the absorbance was determined at 440 nm using a microplate reader

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In the most immediate period after injury induction (skin defect) (i.e., 2, 5, and 10 min), BPC 157 increases Akt1, Braf, Egfr, Egr1, Grb2, Hdac7, Kras, Mapk1, Mapk3, Mapk14, Nos3, Pik3cd, Plcg1, Prkcg, Ptk2, Pxn, Src, Srf, and Vegfa gene expression in rats excision wounds in the skin