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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C
Next, the lysate was centrifuged at 12,000 g for 10 min at 4C
Glutathione also helps regulate cytokine production, the bodys internal signaling system for inflammation
IV infusion delivery method has many advantages over oral supplements: During the digestive process, a portion of oral supplements may be absorbed into the bloodstream
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