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Methods Animals We used 6-week-old male C57BL/6J mice (obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology) and 6-week-old male Nrf2 knockout mice (B6.129 1-Nfe2l2 tm1Ywk /J, purchased from Jackson Laboratory, Bar Harbor, ME) for the experiments

The following protocols represent commonly reported approaches, though individual response varies and conservative starting doses remain prudent

Detoxification Glutathione helps remove toxins and heavy metals from the body, promoting better liver function