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Glutathione is an essential antioxidant and sulfur compound thats composed of three amino acidscysteine, glycine, and glutamic acidand is the major non-protein thiol in most organisms, including humans

Then, the slides were incubated with mouse primary Nrf2 antibody (Proteintech, 66504-1-lg) and rabbit primary PKM2 antibody (Cell Signaling Technology, 4053) at 4 C overnight and then with PLA probe solution for 1 h at 37 C