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Researchers exploring NAD+ optimization often combine 5-Amino-1MQ with precursors like NMN (nicotinamide mononucleotide) or NR (nicotinamide riboside) to address both sides of the NAD+ equation: increasing synthesis while reducing degradation
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CellTiter-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)
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