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Separation of soluble and insoluble components Single mouse hippocampal tissue was isolated, and 300 l mixed protein lysis solution with RIPA (Applygen, C1053), a protease inhibitor (Merck, 539134), and a phosphatase inhibitor (Merck, 524628) (=100:1:2) were added

Old data: 25% of weight loss is muscle mass on GLP-1 drugs like Ozempic New data: It's actually 40% of weight lost is muscle [Edited: was badly phrased as x% muscle loss] Let's put this in perspective: Natural sarcopenia after 40: 3-8% muscle loss per decade = roughly 9-24% over 30 years On GLP-1s: 20-27% muscle loss in 12-18 months Youre losing in 18 months what would naturally take 30+ years to lose