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Band intensities were quantified by densitometry

In addition, we note that in response to comments in the FY 2021 IPPS/LTCH PPS final rule (85 FR 59015 through 59016), we increased the burden estimate from 15 hours to 20 hours after consideration of comments stating that additional effort would be necessary to crosswalk discharges to an MS-DRG, specifically if a hospital is not familiar with the MS-DRG classification system, for use in calculating the median payer-specific negotiated charges

This flexibility allows researchers to produce a final protein product that is free of the affinity tag, which may be necessary for certain functional assays or structural studies