The blood samples were diluted 1:2 in phosphate-buffered saline (PBS), layered onto a separation solution (Biocoll 1.077 g/ml, Biochrom AG, Berlin, Germany), and centrifuged (800 x g, 20 min, 20C, Multifuge X3 F2, Thermo Fisher Scientific, Dreieich, Germany)
Journal of Aging Science
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Indeed, for ILC2s we observed that this concentration did not precipitate changes in cellular viability ( Figures 3F, G ), and was additionally required to produce maximal suppression of ILC2 activity ( Figures 3H, I )