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In male Nrf2 -/- knockout mice, there was a reduction in gene expression levels of antioxidant enzymes in the testis and epididymis, including catalytic glutamate cysteine ligase ( Gclc ), glutamate cysteine ligase modifying subunit ( Gclm ) the rate-limiting enzyme in GSH synthesis glutathione transferase m1 ( Gstm1 ), Gstm2 , Gsta3 , and Sod2 , as well as a depletion in GSH concentration and GPX activity compared to wild-type males

Here are five important takeaways