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After 12 h of incubation under standard culture conditions (37 C, 5% CO, and normoxia), the medium in the NC and HY groups was replaced with fresh complete medium

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The in vivo results from showed that the total H3K4me3 levels were much higher, and those of H2AK119ub were much lower in the hippocampi of BHBA-treated mice as than those of the control mice, which was consistent with the in vitro results (Figures 2C,D)

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