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Watkins PB, Kaplowitz N, Slattery JT, et al

Quantitative real-time PCR (qPCR) for assaying relative expression of TMV-CP and tobacco defense genes ( NtPR1-a , NtPRB-1b , NtGSTtau , and NtGSTphi ) was conducted with the 2SYBR FAST Readymix reagent (KAPA Biosystems, USA)

In this review, we emphasize on (1) GSH depletion as a fundamental risk factor for diseases like chronic obstructive pulmonary disease and atherosclerosis (ischemic heart disease and stroke), (2) importance of oxidative stress and antioxidants in SARS-CoV-2 infection and COVID-19 disease, (3) significance of GSH to counteract persistent damaging inflammation, inflammaging and early (premature) inflammaging associated with cell and tissue damage caused by excessive oxidative stress and lack of adequate antioxidant defenses in younger individuals, and (4) new therapies that include antioxidant defenses restoration