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Because the objective aperture limits how widely distributed the electrons are, areas of the specimen that are thicker or more densely expanded seem darker in the image

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Lastly, the high proportion of female participants evaluated in this study is reflective of the natural history of type 2 diabetes in youth [40] and our study results may not be generalisable to males, although there is no evidence to suggest that there are sex differences in glucose production or response to metformin therapy [41]