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HepG2 cells were seeded onto a cell slide in a 24-well plate and transfected with plasmid vectors according to the transfection reagent instructions
Strati F, Cavalieri D, Albanese D, De Felice C, Donati C, Hayek J, Jousson O, Leoncini S, Renzi D, Calabr A, De Filippo C
On a more experimental level, an IgG Fc variant engineered for improved binding to both FcRn and activating FcRs by introducing six amino acid substitutions (Y296W/K334N/P352S/A378V/V397M/N434Y) has been shown to be as efficacious as IVIg in mouse models of arthritis and ITP [403]
As expected, the hydrodynamic size of NPs, determined by the DLS technique, is significantly higher in comparison with the one obtained by TEM or AFM
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